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destination vector pagm4723  (Addgene inc)


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    Structured Review

    Addgene inc destination vector pagm4723
    Destination Vector Pagm4723, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pagm4723+plasmid/pAGM4723+(Plasmid+%2348015)/pm41923635-215-6-9
    Average 94 stars, based on 45 article reviews
    destination vector pagm4723 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: The new kid on the block: a dominant-negative mutation of phototropin1 enhances carotenoid content in tomato fruits.
    Article Snippet: .. The human codon-optimized CAS9 with 2x35S CaMV promoter and Nos terminator was amplified from pAGM4723 plasmid (Addgene# 49772) using KpnI forward, and PacI reverse primers (Table S11). ..

    Article Title: The new kid on the block: A dominant-negative mutation of phototropin1 enhances carotenoid content in tomato fruits
    Article Snippet: .. The human codon-optimized CAS9 with 2×35S CaMV promoter and Nos terminator was amplified from pAGM4723 plasmid (Addgene# 49772) using Kpn I forward and Pac I reverse primers ( ). ..

    Article Title: Introgression of a dominant phototropin1 mutant superenhances carotenoids and boosts flavor-related volatiles in genome-edited tomato RIN mutants
    Article Snippet: .. The human codon-optimized CAS9 with 2x35S CaMV promoter, and NOS terminator was amplified from pAGM4723 plasmid (Addgene# 49772) using Kpn I forward Pac I reverse primers. ..

    Plasmid Preparation:

    Article Title: The new kid on the block: a dominant-negative mutation of phototropin1 enhances carotenoid content in tomato fruits.
    Article Snippet: .. The human codon-optimized CAS9 with 2x35S CaMV promoter and Nos terminator was amplified from pAGM4723 plasmid (Addgene# 49772) using KpnI forward, and PacI reverse primers (Table S11). ..

    Article Title: Breakthrough in Dicot Prime Editing: Enabling Heritable Desired Edits in Tomato and Arabidopsis
    Article Snippet: .. For the T-DNA-based PE tools, the expression cassettes of the selection marker (NptII, Addgene #51144), PE protein, and pegRNA were cloned into the pAGM4723 plasmid (Addgene #48015). ..

    Article Title: Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis.
    Article Snippet: Prime editing (PE) enables almost all types of precise genome editing in animals and plants.. It has been successfully adapted to edit several plants with variable efficiency and versatility.. However, this technique is inefficient for dicots for unknown reasons.

    Article Title: The new kid on the block: A dominant-negative mutation of phototropin1 enhances carotenoid content in tomato fruits
    Article Snippet: .. The human codon-optimized CAS9 with 2×35S CaMV promoter and Nos terminator was amplified from pAGM4723 plasmid (Addgene# 49772) using Kpn I forward and Pac I reverse primers ( ). ..

    Article Title: Introgression of a dominant phototropin1 mutant superenhances carotenoids and boosts flavor-related volatiles in genome-edited tomato RIN mutants
    Article Snippet: .. The human codon-optimized CAS9 with 2x35S CaMV promoter, and NOS terminator was amplified from pAGM4723 plasmid (Addgene# 49772) using Kpn I forward Pac I reverse primers. ..

    Expressing:

    Article Title: Breakthrough in Dicot Prime Editing: Enabling Heritable Desired Edits in Tomato and Arabidopsis
    Article Snippet: .. For the T-DNA-based PE tools, the expression cassettes of the selection marker (NptII, Addgene #51144), PE protein, and pegRNA were cloned into the pAGM4723 plasmid (Addgene #48015). ..

    Article Title: Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis.
    Article Snippet: Prime editing (PE) enables almost all types of precise genome editing in animals and plants.. It has been successfully adapted to edit several plants with variable efficiency and versatility.. However, this technique is inefficient for dicots for unknown reasons.

    Selection:

    Article Title: Breakthrough in Dicot Prime Editing: Enabling Heritable Desired Edits in Tomato and Arabidopsis
    Article Snippet: .. For the T-DNA-based PE tools, the expression cassettes of the selection marker (NptII, Addgene #51144), PE protein, and pegRNA were cloned into the pAGM4723 plasmid (Addgene #48015). ..

    Article Title: Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis.
    Article Snippet: Prime editing (PE) enables almost all types of precise genome editing in animals and plants.. It has been successfully adapted to edit several plants with variable efficiency and versatility.. However, this technique is inefficient for dicots for unknown reasons.

    Marker:

    Article Title: Breakthrough in Dicot Prime Editing: Enabling Heritable Desired Edits in Tomato and Arabidopsis
    Article Snippet: .. For the T-DNA-based PE tools, the expression cassettes of the selection marker (NptII, Addgene #51144), PE protein, and pegRNA were cloned into the pAGM4723 plasmid (Addgene #48015). ..

    Article Title: Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis.
    Article Snippet: Prime editing (PE) enables almost all types of precise genome editing in animals and plants.. It has been successfully adapted to edit several plants with variable efficiency and versatility.. However, this technique is inefficient for dicots for unknown reasons.

    Clone Assay:

    Article Title: Breakthrough in Dicot Prime Editing: Enabling Heritable Desired Edits in Tomato and Arabidopsis
    Article Snippet: .. For the T-DNA-based PE tools, the expression cassettes of the selection marker (NptII, Addgene #51144), PE protein, and pegRNA were cloned into the pAGM4723 plasmid (Addgene #48015). ..

    Article Title: Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis.
    Article Snippet: Prime editing (PE) enables almost all types of precise genome editing in animals and plants.. It has been successfully adapted to edit several plants with variable efficiency and versatility.. However, this technique is inefficient for dicots for unknown reasons.



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